Back to Blog
Original question: What is the ASHRAE 110 standard?

ASHRAE 110: Understanding the Standard for Laboratory Fume Hood Performance

March 5, 2026

Ensuring the safety and effectiveness of laboratory fume hoods is crucial for protecting lab personnel from exposure to hazardous substances. The ASHRAE 110 Standard, formally titled "Method of Testing Performance of Laboratory Fume Hoods," provides a comprehensive framework for evaluating the performance of these critical safety devices. This article will delve into the details of ASHRAE 110, explaining its components, the rationale behind its testing methods, and its significance in maintaining a safe laboratory environment.

Overview of ASHRAE 110

ASHRAE Standard 110 establishes the accepted procedures for testing the performance of laboratory fume hoods. Developed by the American Society of Heating, Refrigerating, and Air-Conditioning Engineers (ASHRAE), this standard is a cornerstone for ensuring that fume hoods operate effectively to protect researchers from exposure to hazardous airborne contaminants.

The standard outlines three primary tests to assess fume hood performance:

  1. Face Velocity (FM) Test
  2. Smoke Visualization (SM) Test
  3. Tracer Gas Containment (AS) Test

These tests can be conducted in three different conditions: 'as manufactured' (AM), 'as installed' (AI), and 'as used' (AU) with actual equipment in place. Let's explore each of these components in detail.

The Face Velocity (FM) Test

The Face Velocity Test measures the speed at which air is drawn into the fume hood. An anemometer is used to take readings at various points across the hood's face opening. The objective is to ensure that the airflow is sufficient to contain and exhaust hazardous fumes effectively.

Why Face Velocity Matters

Maintaining appropriate face velocity is crucial because it directly influences the containment efficiency of a fume hood. If the velocity is too low, hazardous fumes may escape into the laboratory environment. Conversely, excessively high face velocities can cause turbulence, potentially pulling fumes out of the hood instead of containing them.

Practical Guidance

  • Regular Monitoring: Conduct face velocity tests periodically, as part of routine maintenance, and whenever significant changes occur in the laboratory setup or fume hood configuration.
  • Target Range: Aim for a face velocity between 80 and 120 feet per minute (fpm), as recommended by industry standards, to balance containment and energy efficiency effectively.

The Smoke Visualization (SM) Test

The Smoke Visualization Test involves using a smoke tube to visually assess the airflow patterns within the fume hood. This test helps identify areas of turbulence or reverse airflow that could compromise the hood's ability to contain hazardous substances.

The Purpose of Smoke Visualization

By visualizing airflow, lab personnel can detect potential issues that might not be apparent through velocity measurements alone. It provides a qualitative assessment of how well the hood is capturing and expelling contaminants.

Practical Guidance

  • Conduct Regularly: Perform smoke tests during initial setup and after any major modifications to the laboratory environment or hood configuration.
  • Identify Problems: Use smoke tests to pinpoint sources of airflow disruption, such as obstructions or improper sash operation, and take corrective measures promptly.

The Tracer Gas Containment (AS) Test

The Tracer Gas Test is the most sensitive and critical component of ASHRAE 110. It involves releasing a small quantity of sulfur hexafluoride (SF6) gas inside the fume hood and measuring its concentration at a mannequin's breathing zone. The test aims to ensure that the hood effectively contains and exhausts hazardous gases.

Why the Tracer Gas Test is Crucial

SF6 is used due to its inert nature and detectability at very low concentrations. The test's sensitivity ensures that even small leaks or containment failures are identified, protecting lab personnel from potential exposure to harmful substances.

Practical Guidance

  • Acceptance Criteria: A fume hood passes the tracer gas test if the SF6 concentration at the mannequin's breathing zone does not exceed 0.05 parts per million (ppm) during a 4 L/min SF6 challenge.
  • Testing Conditions: Perform tests 'as manufactured', 'as installed', and 'as used' to account for variations in performance due to installation and operational conditions.

The Importance of ASHRAE 110 Compliance

Adhering to ASHRAE 110 is essential for maintaining a safe laboratory environment. By ensuring that fume hoods perform as expected, laboratories can:

  • Protect Personnel: Minimize the risk of exposure to hazardous substances, safeguarding the health and well-being of lab personnel.
  • Ensure Compliance: Meet regulatory requirements, such as those outlined in OSHA 29 CFR 1910.1450 and NFPA 45, which mandate effective containment of hazardous chemicals.
  • Optimize Performance: Identify and address performance issues, enhancing the overall efficiency and reliability of laboratory safety equipment.

Conclusion

The ASHRAE 110 Standard provides a rigorous framework for evaluating the performance of laboratory fume hoods. By understanding and implementing its testing methods, lab professionals can ensure that their fume hoods function effectively, protecting personnel and maintaining compliance with safety regulations. Regular testing and maintenance, guided by ASHRAE 110, are integral to fostering a culture of safety and excellence in laboratory environments.

Have a follow-up question?

Use our AI chat assistant to get instant answers about fume hood safety.